FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_96.35200000039e43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_96.35200000039e43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650395
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT38770.5960992935062539No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC30070.4623344275401871No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC26900.4135948154582984No Hit
GTACCCGTGCTGCCGCTCGACTTGCATGTGTTAGGC24040.36962153768094774No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC22820.3508637059017981No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG21850.3359496921101792No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC19990.3073516862829512No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG19580.30104782478340086No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT18310.2815212294067451No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG18210.27998370221173285No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT17890.2750636151876936No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT17740.27275732439517525No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG14480.22263393783777552No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC13390.20587489141214182No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG12880.1980335027175793No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC12880.1980335027175793No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT12760.1961884700835646No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT12450.1914221357790266No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG12240.18819332866950084No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT11890.18281198348695793No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG11870.18250447804795547No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA11790.18127445629194566No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT11740.18050569269443953No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC11550.17758439102391624No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC11280.1734330675973831No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA10850.1668217006588304No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC10640.16359289354930465No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10510.1615941081957887No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG10250.15759653748875685No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG10240.1574427847692556No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC9870.15175393414771024No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC9860.15160018142820902No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT9810.1508314178307029No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC9110.14006872746561705No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC9050.13914621114860967No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT9020.13868495299010603No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT8910.13699367307559251No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT8870.13637866219758762No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT8690.13361111324656555No Hit
GTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTA8540.13130482245404715No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG8520.1309973170150447No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA8490.13053605885654102No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC8360.12853727350302507No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG8290.1274610044665165No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT8150.12530846639349932No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA8070.12407844463748954No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC7850.12069588480846255No Hit
GGTTCGCCTCTTGCACCTATGTATTCAGTACAAGAT7810.12008087393045763No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC7680.11808208857694169No Hit
CCGTTATCCGGATCGAGGACAGTGTATGGTGGGTAG7570.11639080866242821No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA7560.11623705594292699No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC7520.11562204506492207No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA7500.11531453962591964No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC7350.11300824883340124No Hit
GAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTTGT7260.11162447435789019No Hit
ATCCGGATCGAGGACAGTGTATGGTGGGTAGTTTGA7080.10885692540686814No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT7050.10839566724836446No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC7050.10839566724836446No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG6860.10547436557784115No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC6790.10439809654133259No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG6740.10362933294382644No Hit
GGCGTAAAGCGCGCGTAGGTGGTTTGTTAAGTTGGA6690.10286056934632032No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC6680.1027068166268191No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT6630.10193805302931296No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA6620.10178430030981173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTG2850.026.8354323
GTACCCG2950.026.4503751
CCCGTGC3000.025.9935364
GAGCCAT2650.023.7823031
CCGTGCT3250.023.5326085
CCGTATG7850.023.115278
ACCGTAT7900.022.9689717
CCACCGT7900.022.7791465
CATCCAC7950.022.6358812
TATGCGC4650.022.57676711
ATCCACC8000.022.4944063
CGTATGC8200.022.1286459
CACCGTA8200.021.9457656
GTATGCG8300.021.86203410
ACTCGGT1450.021.7187393
TCGACTT3750.021.1996217
TTGCATG3850.021.03696422
TCCACCG8700.020.8568824
GTGTTAG3850.020.660128
TGTGTTA3850.020.660127
Produced by FastQC (version 0.11.2)