FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_95.35200000039e0f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_95.35200000039e0f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891103
Sequences flagged as poor quality0
Sequence length36
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT70500.7911543334496686No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC49460.5550424586158951No Hit
GTACCCGTGCTGCCGCTCGACTTGCATGTGTTAGGC36350.40792141873610566No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC35630.3998415446923644No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG31380.3521478437397248No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG29310.3289182058639686No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG25680.2881821742267729No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC25250.28335669389509405No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC24900.27942897734605315No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG24490.2748279379600338No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT24400.27381795370456613No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT23320.2616981426389542No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT22400.2513738591386181No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG21730.2438550874590255No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA19470.2184932605995042No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT19450.21826881965384473No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC19040.21366778026782537No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG18880.21187225270254953No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC18390.20637344953389225No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG18260.20491458338710564No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT18240.20469014244144618No Hit
GGATGTGATGAGCCGACATCGAGGTGCCAAACACCG17890.20076242589240528No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC17850.20031354400108628No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT17740.19907911879995915No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT17640.19795691407166177No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC16790.18841817388113383No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC16260.1824704888211576No Hit
GGTTCGCCTCTTGCACCTATGTATTCAGTACAAGAT15900.17843055179928693No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG15430.17315618957628914No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT15290.17158510295667279No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT15090.169340693500078No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG14940.1676573864076319No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13890.15587423676050915No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT13800.15486425250504152No Hit
GGAAACCTTGGTCTTTCGGCGTGGGTGTTTTTCACA13700.1537420477767441No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC13610.15273206352127644No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG13550.152058740684298No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC13070.14667215798847047No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC13010.14599883515149203No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT12810.14375442569489724No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA12350.13859228394472917No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC12270.13769452016209124No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA12070.13545011070549645No Hit
ATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTT12060.1353378902326667No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC12020.13488900834134773No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC11640.1306246303738176No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA11590.13006352800966892No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG11530.12939020517269045No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC11460.1286046618628823No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA11320.12703357524326594No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT11040.1238914020040332No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG11020.12366696105837371No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT10920.12254475633007632No Hit
CCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGGT10260.11513820512331348No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC10210.11457710275916476No Hit
TTCCTGTACTTCTGGTTATTGCGATGGAGGGACGGA10140.1137915594493566No Hit
GTATAATGGGTCAGCGACTTATTTTCAGTGGCGAGC10110.11345489803086736No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA10080.11311823661237813No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC10060.11289379566671867No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC9970.111883811411251No Hit
GGCTAGGCCAGCCTGGCGTTGGTTGTCCAGGTTTAA9870.1107616066829536No Hit
CCTAAAGAGTAACGGAGGAGTACGAAGGTGCGCTCA9810.11008828384597516No Hit
GGCCTAGCCTTCTCCGTCCCTCCATCGCAATAACCA9760.10952718148182645No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG9550.10717055155240192No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG9500.10660944918825321No Hit
TCTTTTGATGATGCGGTAGAGGAGCGTTCTGTAAGC9470.106272787769764No Hit
ATATTAACCTGTTTTCCATCGACTACGCTTTTCAGC9180.10301839405770152No Hit
CCCTATTAAGACTCGCTTTCGCTACGCCTCCCCTAT9180.10301839405770152No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT8980.1007739846011067No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT8930.10021288223695801No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC8930.10021288223695801No Hit
GTTTTCCATCGACTACGCTTTTCAGCCTCGCCTTAG8920.10010066176412828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGC4950.028.480164
ACCCGTG5100.027.642513
CCGTGCT5100.027.348445
GTACCCG5200.026.261891
CTAGATA408.167219E-626.2589430
GTCTCTT757.2759576E-1226.00885630
TGTTAGG5500.025.09945529
TTAGGAT300.001223906125.00851430
TCTTAAG300.001227917524.99448210
ATCTTAA300.001227917524.9944829
GTGTTAG5600.024.91919728
TGTGTTA5550.024.87333327
GTTAGGC5750.024.7910530
CTTGCAT5700.024.73693521
TTGCATG5800.024.5690622
CGACTTG5800.024.31043618
ACTCGGT2100.024.2817153
GACTTGC5750.024.2609619
CATGTGT5750.023.74454925
TGCATGT5750.023.74055123
Produced by FastQC (version 0.11.2)