FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_94.35200000039dca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_94.35200000039dca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630535
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT47760.7574520050433362No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC23860.3784088115647823No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC21250.3370153916911829No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG18910.299904049735542No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG17910.2840445018912511No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG14480.2296462527853331No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT14290.2266329386949178No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT14080.2233024336476167No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC13760.21822737833744357No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA13270.21045619989374104No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG13150.20855305415242611No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT12800.2030022124069243No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC12570.19935451640273735No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG12410.19681698874765083No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG12240.19412086561412134No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT12190.19332788822190677No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11960.18968019221771987No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC11120.17635817202851545No Hit
TCCTATATCTACGCATTTCACCGCTACACAGGAAAT10980.17413783533031474No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG10900.17286907150277145No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG10600.1681112071494842No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT10400.16493929758062598No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC9930.15748531009380923No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC9760.15478918696027977No Hit
GTACCCGTGCTGCCGCTCGACTTGCATGTGTTAGGC9760.15478918696027977No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC9660.15320323217585066No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG9600.1522516593051932No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT9320.14781098590879174No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT9170.1454320537321481No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCG8730.13845385268066007No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA8510.13496475215491607No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA8270.13115846067228623No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC8210.13020688780162876No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT8170.12957250588785715No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC8140.12909671945252843No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT8140.12909671945252843No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA7990.12671778727588476No Hit
GGATCACAGTCTGTTTGCCGACTCCCCGAAGCTTTT7880.12497323701301276No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT7800.12370447318546948No Hit
GATATAGGAAGGAACACCAGTGGCGAAGGCGACCAC7740.12275290031481202No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC7620.12084975457349711No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA7410.11751924952619602No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG7360.11672627213398146No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC7280.1154575083064382No Hit
ATATTAACCTGTTTTCCATCGACTACGCTTTTCAGC7170.11371295804356617No Hit
CCTTTTGTATAATGGGTCAGCGACTTATTTTCAGTG7130.11307857612979452No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT7080.11228559873757998No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC6980.11069964395315089No Hit
ATGTATCACTGTAGGGGGTAGAGCACTGTTTCGGCT6960.11038245299626508No Hit
ATCGAGGACAGTGTATGGTGGGTAGTTTGACTGGGG6880.10911368916872179No Hit
TATCTAGGTAGCGCCTCATGTATCACTGTAGGGGGT6850.10863790273339306No Hit
GTCGTTACTCATGTCAGCATTCGCACTTCTGATACC6840.10847930725495017No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC6830.10832071177650725No Hit
CTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTG6680.10594177959986362No Hit
ACCATACACTGTCCTCGATCCGGATAACGGACCTGA6680.10594177959986362No Hit
CCCCTATTCGGTTAAGCTCGCCACTGAAAATAAGTC6580.10435582481543451No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC6560.10403863385854868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGA254.2156896E-430.01031528
GTCTCTT603.929017E-1027.51382330
TTGCATG1850.025.13380622
GTCATAT300.001222919725.010581
GCCCTTT300.001223485625.00859527
TACCTAG300.001223485625.00859527
CATGTGT1800.025.0046325
CTTGCAT1900.024.4723921
TGTGTTA1850.024.33268727
GTACCCG2000.024.0101571
GGTCCGA702.3501343E-923.5814061
GTGTTAG1950.023.08485828
TGTTAGG1950.023.08485829
GTTAGGC2050.022.69432830
CCCTATA603.2687058E-722.5095211
CTAGTAA402.4600633E-422.50773628
CGCTAGT402.4613852E-422.50594926
CCCGTGC2150.022.322634
ACCCGTG2150.022.320863
CGACTTG2050.021.9500618
Produced by FastQC (version 0.11.2)