FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_93.35200000039d87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_93.35200000039d87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70280
Sequences flagged as poor quality0
Sequence length36
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT3280.466704610130905No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC2900.41263517359134894No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC2580.36710301650540694No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT2190.31161070005691516No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC1810.2575412635173591No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1520.21627774615822423No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT1510.21485486624928857No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC1430.20347182697780306No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG1380.19635742743312465No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT1340.1906659077973819No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG1280.1821286283437678No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG1240.17643710870802504No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG1210.172168468981218No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT1200.1707455890722823No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1110.15793966989186112No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC1110.15793966989186112No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA1000.14228799089356858No Hit
CCTTTATACTCTACGACCGATTTCCGACCGGTCTGA990.1408651109846329No Hit
CTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACC960.13659647125782584No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC960.13659647125782584No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC950.13517359134889015No Hit
CGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGACT940.13375071143995446No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG920.1309049516220831No Hit
ACCAGGAACCGTACCCCAAACCGACACAGGTGGTTG920.1309049516220831No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG910.1294820717131474No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG900.12805919180421174No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC900.12805919180421174No Hit
GGTACGCAGTCACCCAACAAAGTGGGCTCCCACTGC850.1209447922595333No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC850.1209447922595333No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT850.1209447922595333No Hit
ATTTCCGACCGGTCTGAGCGCACCTTCGTACTCCTC820.11667615253272624No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC800.11383039271485487No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC790.11240751280591917No Hit
CCTTAGTAGTGGCGAGCGAACGGGGACCAGCCCTTA770.10956175298804782No Hit
CCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGT740.10529311326124076No Hit
GTTTCGGGTCTATTCCCAGCGACTAGACGCCCTATT730.10387023335230507No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC720.10244735344336939No Hit
CCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGA710.10102447353443368No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG710.10102447353443368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTAT200.005115995629.99857713
ATTCGTG200.005115995629.99857722
CCATTCG200.005115995629.99857720
ATCGTTA200.005115995629.99857711
CCATCGT254.187647E-429.9985759
CCCACAT350.002979288321.42755512
CCACTTA350.002979288321.42755525
CCACATC350.002979288321.42755513
CACTTAA350.002979288321.42755526
CTTTATA455.3725584E-420.0132962
CCTTTAT455.3725584E-420.0132961
TCCCACA400.00645300818.74911111
GTGCAGG400.00645300818.74911126
CACATCG400.00645300818.74911114
TTTCCCA400.00645300818.74911121
TCGTTTC400.00645300818.74911118
TTAACCA400.00645300818.74911129
CCATACC651.6011589E-518.4738122
TATACTC500.001096532617.9991465
TTATACT500.001096532617.9991464
Produced by FastQC (version 0.11.2)