FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_92.35200000039d44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_92.35200000039d44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129204
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT7010.542552862140491No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC4100.3173276369152658No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC3500.27088944614717814No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT3200.24767035076313426No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC3140.2430265316863255No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG2860.22135537599455124No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC2840.21980743630228167No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2680.20742391876412497No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT2660.20587597907185534No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG2590.20045819014891178No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG2520.19504040122596825No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG2130.16485557722671126No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT2050.1586638184576329No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC2030.15711587876536331No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG1950.15092411999628494No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC1840.1424104516888022No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1830.14163648184266742No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1800.13931457230426303No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG1730.13389678338131947No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA1710.13234884368904987No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG1700.13157487384291508No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG1690.1308009039967803No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1680.1300269341506455No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA1670.1292529643045107No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC1660.1284789944583759No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT1590.12306120553543234No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC1560.12073929599702796No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC1560.12073929599702796No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT1550.11996532615089317No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC1490.1153215070740844No Hit
TCTCTACCCAACCACCTGTGTCGGTTTGGGGTACGG1420.10990371815114083No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG1400.10835577845887123No Hit
GGACCGAACTGTCTCACGACGTTCTAAACCCAGCTC1360.10525989907433206No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT1360.10525989907433206No Hit
CGTTAGCACCCGCCGTGTGTCTCCCATGCTCGGCAC1300.10061607999752331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGCT254.2058152E-430.04
CCGTATG903.6379788E-1223.3333328
TACCCTT1150.022.1739145
CGTATGC957.2759576E-1222.105269
GTATGCG957.2759576E-1222.1052610
ACCGTAT957.2759576E-1222.105267
CCACCGT957.2759576E-1222.105265
GTTAGAA350.002991899621.4285723
TATGCGC706.002847E-821.42857211
AGTTAGA350.002991899621.4285722
CCATACC1200.021.252
CATACCC1200.021.253
GCCATAC1300.020.769231
ATACCCT1250.020.44
AGATCTT400.00647999118.74999820
CTGAGAT400.00647999118.74999817
GTAGGCT400.00647999118.74999812
ACCCTTT400.00647999118.74999827
ATCTTAG400.00647999118.74999822
CCCTTTA400.00647999118.74999828
Produced by FastQC (version 0.11.2)