FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_91.35200000039d00.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_91.35200000039d00.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113705
Sequences flagged as poor quality0
Sequence length36
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACCCTTGGGACCGGCTTCAGCCCCAGGATGT9950.8750714568400686No Hit
GCATCCACCGTATGCGCACAAGCAGTGGGAGCCCAC4630.4071940547909063No Hit
GTGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTC4620.406314585990062No Hit
CCCTTGGGACCGGCTTCAGCCCCAGGATGTGATGAG3370.29638098588452577No Hit
GTCTAAGCCTTCCCACATCGTTTCCCACTTAACCAT3340.29374257948199284No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCG3180.27967107866848423No Hit
CTTTAAAGGGTGGCTGCTTCTAAGCCAACCTCCTAG2850.2506486082406227No Hit
CCGCTAACTTTTCAAACCGATGCAAACTCCGAATAC2840.24976913943977838No Hit
GGTCTATTCCCAGCGACTAGACGCCCTATTAAGACT2730.2400949826304912No Hit
CAGCGCCGCCATCGTTACGCCATTCGTGCAGGTACG2640.2321797634228926No Hit
GGCTTAGACAGCTAGGAGGTTGGCTTAGAAGCAGCC2560.22514401301613826No Hit
GCATCCACCGTATGCGTACAAGCAGTGGGAGCCCAC2470.21722879380853966No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGT2420.21283144980431817No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTAC2340.2057956993975639No Hit
ATTAAGACTCGCTTTCGCTACGCCTCCCCTATTCGG2170.19084472978321093No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2090.18380897937645663No Hit
CTCTACGACCGATTTCCGACCGGTCTGAGCGCACCT2070.18205004177476805No Hit
CTATCTAGGTAGCGCCTCATGTATCACTGTAGGGGG2000.17589376016885802No Hit
ACCTACAAGTGCCGAGCATGGGAGACACACGGCGGG1910.1679785409612594No Hit
GTATAAAGGCAAAAGCGCGCTTGACTGCGAGACAGA1910.1679785409612594No Hit
ACCTAAATAGCTTTCGAGGAGAACCAGCTATCTAGG1840.16182225935534938No Hit
GTACGAAGGTGCGCTCAGACCGGTCGGAAATCGGTC1810.1591838529528165No Hit
AGCAGTGGGAGCCCACTTTGTTGGGTGACTGCGTAC1800.1583043841519722No Hit
CCTTAAACCTGGACAACCAACGCCAGGCTGGCCTAG1640.14423288333846357No Hit
CGCTAACTTTTCAAACCGATGCAAACTCCGAATACC1550.13631766413086496No Hit
ATACAAAAGGTACGCAGTCACCCAACAAAGTGGGCT1450.12752297612242205No Hit
GAGCCATGATCAGTCACACTGGAACTGAGACACGGT1400.12312563211820061No Hit
ACCATACACTGTCCTCGATCCGGATAACGGACCTGA1390.12224616331735633No Hit
ATACAGAACCACCGGATCACTAAGACCTACTTTCGT1370.12048722571566774No Hit
CCTTCGTACTCCTCCGTTACTCTTTAGGAGGAGACC1360.11960775691482345No Hit
GTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTT1350.11872828811397916No Hit
GGTTTAAGGTGGTAGGCTGAGATCTTAGGTAAATCC1340.11784881931313487No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAA1340.11784881931313487No Hit
GTCGTAGAGTATAAAGGCAAAAGCGCGCTTGACTGC1330.11696935051229058No Hit
GCTTCAGCCCCAGGATGTGATGAGCCGACATCGAGG1320.1160898817114463No Hit
GCGAACAGCCATACCCTTGGGACCGGCTTCAGCCCC1320.1160898817114463No Hit
GGCTTACAGAACGCTCCTCTACCGCATCATCAAAAG1280.11257200650806913No Hit
CCTCCTAGCTGTCTAAGCCTTCCCACATCGTTTCCC1270.11169253770722484No Hit
GCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAA1250.10993360010553627No Hit
TGCGCACAAGCAGTGGGAGCCCACTTTGTTGGGTGA1220.10729519370300337No Hit
CTTTGGGACCTTAGCTGACGGTCTGGGTTGTTTCCC1210.10641572490215909No Hit
CTATTAAGACTCGCTTTCGCTACGCCTCCCCTATTC1180.10377731849962624No Hit
GAACTCGGTCGTAGAGTATAAAGGCAAAAGCGCGCT1170.10289784969878195No Hit
CTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTAC1160.10201838089793763No Hit
CTTCCATACAGAACCACCGGATCACTAAGACCTACT1150.10113891209709334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAGCA300.001218305325.00513527
CGGGTCT300.001221436524.9941375
GGGTCTA300.001221436524.9941376
CATCCAC957.2759576E-1222.1000792
CATACCC1650.021.8130633
GAAGCAG350.002986148221.4329728
TTCGGGT350.002993785821.4235463
ATGCGCA706.0017555E-821.42354612
TAAGCCA859.349606E-1021.18081721
GCCATAC1800.020.8376081
ATACCCT1750.020.5666054
CCATACC1850.020.2655162
CTATTCG608.129933E-620.00410729
AAGCCAA901.904482E-920.00410522
GCATCCA1052.910383E-1120.0041051
TACCCTT1800.019.995315
CCTGTTA608.160949E-619.995318
TATGCGC751.2436612E-719.9953111
CCGTATG1200.019.995318
TGTTATC608.160949E-619.9953110
Produced by FastQC (version 0.11.2)