FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_89.35200000039c95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_89.35200000039c95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672996
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10060.1494808290093849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCG1251.8189894E-1219.193969
CGGCCCG1353.6379788E-1217.7761514
CCCGGCC1353.6379788E-1217.7761512
AGCCCGG1353.6379788E-1217.77218810
GCCCGGC1450.017.58328811
CCCGGAG1501.8189894E-1216.99970617
GGAGCCC1451.0913936E-1116.5465188
CGGAGGG1551.8189894E-1216.45132619
CCGGCCC1501.8189894E-1115.99853313
AGGAGCC1700.015.8773577
GCCCGGA1655.456968E-1215.4531316
GGGCCCG1608.0035534E-1014.06330123
TATACCG1402.9936928E-813.92419055
CAGGAGT650.00639573513.84184
CCGGAGG2009.094947E-1213.49976518
AAGGAGC2009.094947E-1213.4957536
CCCGGGG1251.767723E-613.20369526
GTATAGG1855.020411E-1012.977571
GCCCGGG1753.0468073E-912.86074325
GTGAGTA954.1945276E-412.6276069
Produced by FastQC (version 0.11.2)