FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_88.35200000039c52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_88.35200000039c52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973228
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18820.193377091493463No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCCG350.003008127721.4257459
TAGTGCG550.002104629516.3597977
CTAAGGC1006.46013E-411.9971853
CGGTGTT2401.8189894E-1111.8734349
GTCTTAA1151.5693117E-411.7460321
ACGGTGT2301.05501385E-1011.7375828
TATACGG2950.011.6921725
TATACCG1551.5465612E-611.6101795
TACGGTG2503.8198777E-1111.3973257
GTCTATA1859.0709364E-811.3580241
AAGACCG1202.348513E-411.2473615
TTAGCGA950.006005965611.0500386
TTACCCC950.006005965611.0500384
GTACTAG1551.9403946E-510.651421
AAACCCT1551.9581164E-510.6426645
GTTGTGC1000.00879073310.4975376
CTGGCCT1000.00879073310.4975374
ACTGTCC1451.1675473E-410.3434638
ATACGGT2905.2750693E-1110.3424016
ACTATGT1602.7767035E-510.311148
Produced by FastQC (version 0.11.2)