FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_87.35200000039c1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_87.35200000039c1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157848
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18690.16142015186794814No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGA550.002097171516.36886826
CCCGCAT650.006372574713.85058127
TAGGACT1252.5789172E-511.9976164
GTAGGAC1059.727056E-411.4263013
GTCTAAG1859.100404E-811.3559631
TCGATCG950.006005521411.0504369
GGTAGTC950.006005521411.0504367
ACCCTAT1100.001427848210.9135231
TTAGACT2207.761628E-910.9069244
TAGAACA2402.6575435E-910.62288954
ATCGCCC1000.00877321210.50063423
TGAACCG1000.00879008210.4979145
TAGACTT2301.5523256E-810.4327095
TACCTAG1150.002078200510.4327095
GTCTAGA1304.896012E-410.3888341
GTATAAA3900.010.3888331
GTGTATA1653.8747996E-510.0040621
TTAATAC3650.09.8610553
GTATTAG2454.0978193E-89.7998981
GTATATC2301.7158527E-79.7865821
Produced by FastQC (version 0.11.2)