FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_86.35200000039bd9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_86.35200000039bd9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040728
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17230.16555718689225235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCAG1154.0827217E-815.6493679
TAGGTAG1555.002221E-1014.5135265
GCCGCGT1006.436961E-412.002460525
CGCCGCG1006.436961E-412.002460524
AAGACTC1006.457795E-411.9978485
TATACTG3900.011.5363915
GGTAGTC1755.4824704E-711.1408597
GACATAG1901.319222E-711.05489930
GACAGCC1100.001434631110.9071347
CTAGGAC1100.001434631110.9071343
ATCTTAG1807.827002E-710.8376381
TCGCCGC1253.420346E-410.800137523
TATAGGG1253.4249094E-410.7985812
CTAATAC2802.7284841E-1110.7123643
AGGTAGT1851.121969E-610.538656
CCCCTAC1000.008750332510.5041721
GGCCTAT1000.008750332510.5041721
CTGTCTG1000.00878782310.49811659
ATACTGT3203.6379788E-1210.310656
CTAAAGG1602.7786658E-510.310653
Produced by FastQC (version 0.11.2)