FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_85.35200000039b96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_85.35200000039b96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430044
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8360.1943987126898643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAA653.4309688E-416.1534712
GCACAAT750.001015411314.0013031
TTCAGGA750.001016304713.9996752
TATAGGG650.006378462513.8458322
GTCATAT850.00260585512.3540921
TGTTATA900.00400080911.6663952
CTATTCT1253.4163488E-410.7984924
TATAATA2955.456968E-1210.6777182
TGTAGTA1150.002070610210.434542
TCTATTC1150.002072614410.4333263
ATTATAG1653.882634E-59.9986043
CAATACT1501.6398604E-49.9986044
ATAAACC2157.205217E-79.7660793
TATAGAT1250.0041321349.5997762
AATACTA1450.00133779639.3079625
TCCAATA1500.00181826568.9997912
ATATAAT2854.3564796E-88.9482011
TTAATGT1350.0077908788.8876494
TATTATA2403.2077587E-68.7497972
TATACAA1901.8139897E-48.6819895
Produced by FastQC (version 0.11.2)