FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_84.35200000039b53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_84.35200000039b53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710339
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9610.13528751765002345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATA805.1678417E-616.8748822
TAGTCAG706.0396077E-414.9977839
AACTAGG650.00638615613.84508216
CTAAGAC902.6530286E-413.3313623
GGTCTAG800.00165190313.1286041
CTAAGCA800.001657166613.123064
GGTAGTC800.001657166613.123067
CCTATAC1302.7587412E-612.6904323
TAAACGG954.1891425E-412.6297125
GTATTAG1951.1550583E-912.3110721
AGTCAGA1006.4516376E-411.99822510
CTAATAC2401.8189894E-1111.8732443
GTATAAC1652.5397094E-711.8214281
CTATGCA1551.543005E-611.6111869
TAGGTAG1059.7148307E-411.4268825
CTATACT2155.3969416E-911.161144
TACCACG950.00599938411.0509987
ACCACGT950.00599938411.0509988
GTACTAA1100.001428236610.91208651
CTCTAAG1100.001431598310.9090152
Produced by FastQC (version 0.11.2)