FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_83.35200000039b02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_83.35200000039b02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824096
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12930.15689919621015028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC400.00651375918.747618
TAGTCAG1004.2727497E-513.498289
CCCTTAC902.642496E-413.3381081
GCCGTCA850.002613676212.35136713
GGTAGTC1101.0434482E-412.2711637
GTATTAG2503.6379788E-1212.0042981
TAGACTC1006.4524636E-411.9984715
TAGAGTG1059.716058E-411.4271155
TAAGACC1059.716058E-411.4271154
GGACAGT1355.6500598E-511.1096956
ACTGCCG950.005999853411.0512248
ATGACTC1100.001433460510.9077015
GTTATAT2802.5465852E-1110.7181231
TAGGTAG1408.177277E-510.7129215
ATTAGAC1704.6771456E-610.5868853
GTAGACT1000.00878183510.4986624
TATAAAG3600.010.41533852
TTAGACT1304.91693E-410.3832924
GTACTGG1451.1612766E-410.3485331
TAATACT4250.010.233994
Produced by FastQC (version 0.11.2)