FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_81.35200000039a8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_81.35200000039a8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356219
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA254.2134826E-430.0085661
ATTAGAC500.001109189517.9975573
GTATTAG854.681442E-717.6520981
CTTATCC550.002099717316.3614163
GTCTTAT600.003740037615.0042831
CAAGTGT600.00375053614.9979656
TAGCACT600.00375053614.9979654
TCTACCC600.00375053614.9979653
TATTAGG706.0285797E-414.9979652
AGTACAG750.001016567513.9980995
TACTCTG650.006380381513.8442755
AGCCTGT650.006380381513.8442756
TACAGTA650.006380381513.8442752
AATACTA902.6469465E-413.3315245
AGATATA800.001648953913.1287471
GTATAGG800.001648953913.1287471
TTATAGC800.001654194113.1232192
AGAACAG1201.6878677E-512.4983045
GTATTAT850.002600582312.3564691
TAGTAGT850.002608814512.3512654
Produced by FastQC (version 0.11.2)