FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_80.35200000039a47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_80.35200000039a47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244521
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4780.1954842324381137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTCC300.001227155124.9882418
GTATTAT652.7657734E-823.0755081
GACTAAA350.003000531821.4272561
ATCTACA400.006498479318.7488481
CTGTCCA400.00651385118.741189
ATACTCT802.488996E-718.741186
ATACAGT601.8648054E-417.4917686
TGTTCTG550.00210187916.355945
ATTATAG653.4350847E-416.1462483
GTTCACC600.003739102515.00214811
GTATAGG809.707336E-514.9990791
TTAATAC600.003754407414.9929453
TTAGAGT600.003754407414.9929454
ATACACA600.003754407414.9929454
TATTATA1054.2574375E-614.2789952
CCAATAC750.001017537313.9934143
ATATACT1301.819335E-713.8396424
TTATATA1201.1178272E-613.7435322
TATACTC1106.889706E-613.6299515
GTATACA1056.711449E-512.8563541
Produced by FastQC (version 0.11.2)