FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_79.352000000399f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_79.352000000399f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673818
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13170.19545337168196752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG953.8435246E-918.9452135
TAAACCG706.037476E-414.9982935
TATACTG2350.013.402735
ATACTGA1958.367351E-1113.0754366
TAGTCAG1302.7570914E-612.6908639
GACTTAA954.17174E-412.6357671
GTATAAG1907.6579454E-1012.6357671
CTGGGAC1201.68762E-512.50228929
GACATAG1201.6889075E-512.50136130
GTCCTGG850.002603842212.3570371
CCTAATA1702.8196155E-812.3524522
GCTGGGA1101.03988255E-412.27497528
TATTAGG1601.7091043E-712.1870172
GTCCTAA1006.424934E-412.003981
AGCGTGT1006.4450107E-411.99952510
GGTAGCC1006.449032E-411.9986358
ACCTTGC900.00400754811.6653398
GAATAGA1303.8337228E-511.53971825
GTATAGA2103.7034624E-911.4323621
TGGGACT1202.3390066E-411.251224530
Produced by FastQC (version 0.11.2)