FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_78.352000000399b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_78.352000000399b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217385
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40570.18296326528771503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACCG954.1940532E-412.6301829
GTATAAT8550.011.5831531
GTATTAG5500.010.6402271
TATACTG9400.010.5307515
TAGACAG3350.010.297375
ATTATAC8300.010.1193643
CTAATAC5800.010.0850933
CTATACT5950.010.0829194
CGCCGCG1200.002951787610.00160124
CGCCGAC1501.6460012E-49.99912113
GAGCGCA1356.967637E-49.9988959
CTTAGGG1200.00295841469.9988953
TATAATA14100.09.8925242
TAATACT9000.09.8322474
ACTGTGC2454.1402018E-89.7948368
ACTGCCC1409.726702E-49.6417928
GTCTAGT2651.3425961E-89.6261361
CTAGACT2651.3531462E-89.6215784
ATACTGT7650.09.4107256
GGACTGT1603.1513383E-49.3739636
Produced by FastQC (version 0.11.2)