FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_77.3520000003997e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_77.3520000003997e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543528
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15780.10223332521340721No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCG600.003759718514.9969879
CCGTCCT850.002616901612.350469
TAGGTAG1901.0462827E-811.8397265
TTAGGAC1652.561883E-711.8161923
CTCGCGT900.00401319611.664700513
CGGGAGC1501.3557734E-511.00313926
CGGCATA1551.9496332E-510.64785325
AGGCCCC1150.002078912510.43268710
TAGCCTG1756.6291377E-610.28364855
TACAGGC1901.5824407E-610.2610967
GTATTAG4250.010.2378821
GTATTAA4700.010.2153481
TCTTATA4300.010.11490252
CGCCGCG1200.00294944810.00220424
TACGGTG2403.0097E-89.9979927
GTCTTAT2609.838914E-99.8101721
GTGTAAG2454.112553E-89.7983941
ACGGTGT2651.35351E-89.6207098
GTCCTAA2054.1546555E-69.51461
TATACGG3851.8189894E-129.3487715
Produced by FastQC (version 0.11.2)