FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_76.3520000003992e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_76.3520000003992e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407396
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6320.15513161641253229No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACC350.003004073221.42708211
CTTATAA706.016965E-415.002641
TTCGCAA600.003750117714.99895610
CTGTACT650.006379661613.8451914
TATACAG2250.013.3324065
TACATCG800.001654080213.1240875
TTAGACT954.1795708E-412.6306994
CAATGTA1201.688128E-512.499139
ACGGTGT850.002608629912.3520828
ACAGGCA950.00598845511.0518628
ATACGGT950.00598845511.0518626
GTTCTAT1253.4055582E-410.8019011
CTATACT1000.00876527910.499274
GCACAGA1000.00876527910.4992714
ATGGTGC1000.00876527910.499278
TATACGG1150.002071005810.4340575
TTACATC1150.002071005810.4340574
TGAAGCT1304.90288E-410.3838934
TATACAC1304.90288E-410.3838933
TAATACT1756.580234E-610.2849994
Produced by FastQC (version 0.11.2)