FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_75.352000000398e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_75.352000000398e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2961558
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41050.13860947514787825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGT600.003748349415.00446828
TAGGCTG1755.497641E-711.1405315
CCATGCG1100.001436340310.9068149
TAGACTG4000.010.8727295
GACTGCG1551.9612824E-510.6429387
GGACAGT2803.0195224E-1010.1764466
GACAGTC2403.015157E-89.9979117
TCTATAC8700.09.8255343
TATACAG9500.09.3138455
GTATAAG8550.09.3022721
AGGACAG2601.0372423E-79.2288425
CTAGGAG1952.3958326E-59.2288423
ATACCGT3501.9645086E-108.9981216
ACTGCGT2003.2288255E-58.998128
GTCCTAT2854.3855835E-88.9512431
AGACTGC2352.4391866E-68.9343046
ACAGCGG1851.3645011E-48.9170578
CCTATAC4750.08.840263
GTCTTAT5450.08.8111531
ACTGATC6500.08.7674018
Produced by FastQC (version 0.11.2)