FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_74.352000000398a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_74.352000000398a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1388885
Sequences flagged as poor quality0
Sequence length36
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23550.16956047476932937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGACG600.003756574614.9987214
GTACTAG1702.8127033E-812.3576731
ACATCGC850.00261440412.3518898
CATGTAC1501.0405674E-611.9989783
TAATACG2207.754352E-910.9081614
TTAGGGT1807.8830635E-710.8324114
ACAGGGC1000.00878417110.4991068
ATTACAC3009.094947E-1210.4991053
CCTAGAC1150.002076688210.4338943
ACTGACC1304.919569E-410.3837318
GTCCTAT1451.1622963E-410.348791
GTATATA7700.010.32862951
ACAGATC2053.776022E-710.243038
GTATTAG4150.010.1243571
ATAATAC9000.09.9991483
GAGTAAG2157.203471E-79.7711831
TTATACA5850.09.742764
ACTATAC3254.5474735E-119.6914823
CTACTCC1552.2888814E-49.6765954
GTATAAC2505.6055796E-89.6036771
Produced by FastQC (version 0.11.2)