FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_73.35200000039862.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_73.35200000039862.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679631
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16870.24822293273850074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGA854.7089634E-717.6477952
GTCCTAT706.0102734E-415.0072511
CTGTAGA650.00638696913.8446944
GTATATA2009.094947E-1213.5065271
GTCTTAG902.6386295E-413.3397791
TCCTACA800.001654675213.1255462
TATACTG1056.7485365E-512.8557865
TTATACT1302.756933E-612.6909694
TTTATAC1302.756933E-612.6909693
TATATGA954.182036E-412.6321062
AGGTGTC954.1872307E-412.6302467
GTACTAG1101.03699604E-412.278661
TGGACTG1354.2055817E-612.2209335
AATACTT1151.578129E-411.7378925
GTATTGG900.00398976811.6723061
GTACTAT900.00398976811.6723061
TAGACGG1059.710439E-411.4273665
CTGGACT1202.3437201E-411.2488144
GAACAGT950.00599703911.0514666
GTGTAGA1100.001425613410.9143651
Produced by FastQC (version 0.11.2)