FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_72.3520000003982f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_72.3520000003982f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89
Sequences flagged as poor quality0
Sequence length36
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22.247191011235955No Hit
GTGGTGGGGGGGGCGGGGGGGGGTGGGGTGGGGGGG11.1235955056179776No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAG11.1235955056179776No Hit
TACTTAGTGGGTAAGTTTTGTTGAAACTCGAAATAC11.1235955056179776No Hit
GAATATGATTAGAGTCTTCAAAAGATTTTTGGATAT11.1235955056179776No Hit
GGATATATTGATATTAATATCAAACATCACGTAGAA11.1235955056179776No Hit
TCACAAGAGTCTTTAAATAAAAAATTCAACTAGATA11.1235955056179776No Hit
CAGAAGCAGACGACTGGTCGTGAAGAGAGGATAAGC11.1235955056179776No Hit
AGGGGGGGGGGGGGGAGGGGGGAGTGGGGGGGGGGG11.1235955056179776No Hit
CGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGGGGG11.1235955056179776No Hit
AGGTATAAGTGATTTCTTGGGCAATTTGATTGACAA11.1235955056179776No Hit
GGAATGGACAGGAATAAGTTATGGGAGGTAGGAGGG11.1235955056179776No Hit
GGGTGGGAGGGGGGGAGGGGGGGGGGGGGGGGGGGG11.1235955056179776No Hit
CAACTAAACGTTAAAAAATAAAAGTTAAACTAAAAT11.1235955056179776No Hit
CCAAATGTCTTCTTCGTCTGGCTCAACCTTATCATC11.1235955056179776No Hit
AACCTGGACAATCCAATAACTTCTAAAGAGATAAGC11.1235955056179776No Hit
GTGTTACGATAGAAAGCATCAATCGCTCGTCGTTGG11.1235955056179776No Hit
CGGGCATATCATCCAATGATTTCCATATATTACTTG11.1235955056179776No Hit
GTATTGAAAAGAATACAGTGGGAAAATGGACAGAAA11.1235955056179776No Hit
ACTCAAGAAGTAACGCTGACAATTGATGGAATTACA11.1235955056179776No Hit
CATCTATCTCTGGAAGCGACACGGATAGGGACAAAA11.1235955056179776No Hit
CCCGGGGGGGGGGGGAATGGAGTTGTAAGGGGGGCG11.1235955056179776No Hit
GTTGGGGGGACGTGAAGGATTTGGTGCTGATGTTGA11.1235955056179776No Hit
TTCCAAATAAAGTGTGTCTAGCGGCTGCAAGTTGTG11.1235955056179776No Hit
GCCATATATTTAGAATAGACCTAAATAATAATAAAA11.1235955056179776No Hit
GTACTTATATGCGTCTATTTATGTAGGATGAAAGGT11.1235955056179776No Hit
TATCAGAGAAGCAAAAAAGAAGGGGAGAGCTGGCAA11.1235955056179776No Hit
CTGATTTAATGAGCCATTCGCAGTTTCACTGTATAA11.1235955056179776No Hit
GAGTGGCCGTCGGTTATTGTTGTAGTGGGTGATGTT11.1235955056179776No Hit
TTGACGGTATGCAAGTATAAATAAGAAATTCATCGA11.1235955056179776No Hit
CAATCAACATTCTATGTTATGAAAATGGCAAACCAT11.1235955056179776No Hit
TATCATTTGCACTGCACCTATGGTGAAATATCAAGT11.1235955056179776No Hit
AATTAGGAGAGACCAGAAATAATAGACAAAACGATT11.1235955056179776No Hit
CATCTACAACAAATCAAAAGACTGCTAACTAAAGCA11.1235955056179776No Hit
CAAAATATCAAAACCAAATTATCATTAACAGAAGAA11.1235955056179776No Hit
GATATGACGATTGAAATGTCAAGAAAAAAATAATTA11.1235955056179776No Hit
AATTAAAAAGCAATATAAAAAGAAACCAATAGGATG11.1235955056179776No Hit
TAATTCATGAATACATTTCTAAGTTTGTGGAAAGAG11.1235955056179776No Hit
ATACTAGATGTTCTCCTCGAGGATCTACGAATCCTC11.1235955056179776No Hit
TACCGGGCCACGAAAGTAGTGAAAAGGGGAGGGCTA11.1235955056179776No Hit
GCAACGGAAAGAGAATTACAAAAAGTAACGAATAAA11.1235955056179776No Hit
GGCATACACATTGGGGACAGACAAAGGATTTGAGGT11.1235955056179776No Hit
ATTATACACCGCTGCCGCTGACGACGAAAAGAGAGG11.1235955056179776No Hit
GTTAAACTTCAATAAGGATGGTATATTTGATACGTC11.1235955056179776No Hit
GTATAGAGACTAGGCAGATCTGACGATCACCTAGCG11.1235955056179776No Hit
CTATTGAACATTGAACAAGCAAGTTACTGGGCAACA11.1235955056179776No Hit
TGACTAGCGAAGGCGAACTAAACAAGAAGTCGATTA11.1235955056179776No Hit
CACTAATTATTATTTGGTAAAATTTCTCTATCTAAC11.1235955056179776No Hit
GGGATAACTGGCTTGTGGCAGTCAAGCGTTCATAGC11.1235955056179776No Hit
GATAAGAAGTGTCTGTGATGGAGTAGCAAAAAGTGT11.1235955056179776No Hit
CTGTAATTTACTAATCAGCATAATATACCACTACTA11.1235955056179776No Hit
GAACATAGTGCTGCACGATTACGATCTTGCTGATAA11.1235955056179776No Hit
CGGAGATGTGTATAAGAGACAGGCAAAAGATGATGC11.1235955056179776No Hit
GTCGGGTAGTGGGGGCATTGGTCAGAAGCAGCGGGC11.1235955056179776No Hit
CTGAACAGCAATGGGTCGACTAAAAATATTATCCAG11.1235955056179776No Hit
ATGTCAAGTTTGTGAACATACCTCCACAACTTACCA11.1235955056179776No Hit
AAAATCAGAATCAAAAAGACAGCGGTCGGAAGATAG11.1235955056179776No Hit
GGACAGATCACATTATCCAATGCTGTAACCAAAGTA11.1235955056179776No Hit
GTGATTTATGGCAAGATGAAGTCAAGCGAGCATTTC11.1235955056179776No Hit
TAGCTGGGGGGTCGGACAATATGGGGGTAACAAGGC11.1235955056179776No Hit
GTGGTGGCCGTGGGGGTTGGTGGGGGTGGGGGGGTG11.1235955056179776No Hit
ATGGTGATATCGTTCGACGATACAGAGTCAATAAGC11.1235955056179776No Hit
GAGGAGAATATACCGGCATATACTGAGATAGCTCGT11.1235955056179776No Hit
CAATTAAGTTCGTTACATTTACAAGAATGGTCCACC11.1235955056179776No Hit
GGTATGAGCAGGGAGAGAGGCGGAAGAAGGGGGAGG11.1235955056179776No Hit
CTGTTATGTAAGGGTCAATGCCCTGAACCAAAGAGG11.1235955056179776No Hit
GGCAGGAGCAGGGGCCTAGATTAGAGAGAAGTAGAA11.1235955056179776No Hit
GCCAATCGCCGTGCCAAGCATGTAAATCGGTGTGAC11.1235955056179776No Hit
TCCGAGAGTCCTACAAAAAAAAAAAAAAAAAACAGA11.1235955056179776No Hit
TCCCCTCTAATTACATCCATATGCGAATAATTCGAA11.1235955056179776No Hit
AATATGAGGCGAAAAGGGGTGCTTTTGAATTAAATC11.1235955056179776No Hit
GGGGGGGGGGGGAGGGGTGAGTGGGTGGGCAGGAGG11.1235955056179776No Hit
GGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGG11.1235955056179776No Hit
TGCCTGAGCAACATTGGAATAAAGAAGATACCTTTG11.1235955056179776No Hit
TCACAAGAATGACTCGGAAGATGGGCGCTGGAGGGA11.1235955056179776No Hit
GATATGAGGAGGTGTAAGAGTAATAATGGAAGGAGA11.1235955056179776No Hit
GGGTGGGGGGCGTGGCGGGGGGAAGTGGTGGGGGCG11.1235955056179776No Hit
AATATATAACAAGGAAACAAAATATACAATCAATCA11.1235955056179776No Hit
CGTTTAGCTTGAAATATCGAATAGCAGAGACTTCTA11.1235955056179776No Hit
TTATGTAGAAGGGTTGAGTTAGGGGGAGGTTGGGTA11.1235955056179776No Hit
TGGGGGTTGATGATAATGATGCACGAGGCGACGAAG11.1235955056179776No Hit
GCTCATCTCTTCTCCATAATGGTGTTGTAGAGGTTA11.1235955056179776No Hit
AATCAGGGGAAGGTGAGGTGGGTGGAGGTGCAATTA11.1235955056179776No Hit
GAGGATGATCTTCATGGCGGAAACTTGCAGTATTCC11.1235955056179776No Hit
GGGTAGTGTAGGGTAGAGTGCGGGTGGTCTAAGAAG11.1235955056179776No Hit
GGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG11.1235955056179776No Hit
GTCATGAATTGCCCTCAGAGATCTTTATATTCTTGT11.1235955056179776No Hit
GGATAGTTCGTGGGGAGGGGTAGCTGGGAGAGAAAG11.1235955056179776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATTG50.029.9999984
GATATTA50.029.99999810
AAACATC50.029.99999822
GATATAT50.029.9999982
CACGTAG50.029.99999828
ACGTAGA50.029.99999829
CGTAGAA50.029.99999830
TGATATT50.029.9999989
CATCACG50.029.99999825
TATTGAT50.029.9999986
GGATATA50.029.9999981
ACATCAC50.029.99999824
TCACGTA50.029.99999827
TCAAACA50.029.99999820
ATTAATA50.029.99999813
ATTGATA50.029.9999987
TTGATAT50.029.9999988
TATCAAA50.029.99999818
TAATATC50.029.99999815
ATCACGT50.029.99999826