FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_70.352000000397a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_70.352000000397a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538757
Sequences flagged as poor quality0
Sequence length36
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9470.17577497832974792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAACG400.006512738318.7466934
TCATAAC400.006512738318.7466933
CCTATAC755.620445E-515.9971783
TACACTC1002.572062E-614.9973555
ATTAACC600.003754876814.99735453
TATTACA600.003754876814.99735452
TTACACT600.003754876814.99735454
TCCTATA851.6333483E-414.1151572
TACACCG851.6333483E-414.1151575
GATTATA750.001015215314.0027291
GACTGAT650.00638770213.8437137
ATTAAAC954.187231E-412.6293523
ATACTGC850.002612608512.3507626
ATAGTGT850.002612608512.3507628
TAAGAGT1006.448725E-411.9978854
ACTGGAT1006.448725E-411.9978853
GTATTTA900.00399653411.66894151
ATCTATA900.00399653411.66894151
TCTTATA900.004007638411.6646092
TAGCACC900.004007638411.6646094
Produced by FastQC (version 0.11.2)