FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_67.352000000396de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_67.352000000396de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794791
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12280.15450602737071759No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC402.4683314E-422.497643
TACACAG703.1132702E-517.141065
GGACTGA550.002102348516.3619216
GTCTAAG706.02148E-415.004091
GATCCTC706.038758E-414.9984276
GTCATGC650.006388213.8447028
TGTCCAC650.006388213.84470210
TTATGCC800.001656932813.1236243
TGTACTG1151.0931182E-513.04210955
ACTATCC954.188597E-412.6302543
CTAAGAC850.002613093512.35164453
ACAGATC1006.450796E-411.9987418
TATAGAC1901.0415533E-811.8408633
ACTGTCC1151.5787657E-411.7378998
CTATCCT1059.7135536E-411.4273734
ACTTTGC1059.7135536E-411.4273738
CTAGTAC950.005998527611.0514723
ACTACAC950.005998527611.0514723
ATAATAC3550.010.9847633
AATACCG1653.2704975E-610.9079475
Produced by FastQC (version 0.11.2)