FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_65.35200000039658.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_65.35200000039658.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776007
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGA600.00376104814.9963684
TAGGACT1302.7682909E-612.6892354
GTGTTAG1202.3397227E-411.2529781
TAGACCG1355.665192E-511.1084215
TTCTATA2301.3169483E-911.0852082
TTATACT3400.011.0267414
GTATTAG2504.5110937E-1010.8028591
CGTATAC2251.1048542E-810.66408353
TCAGGAC1756.6342254E-610.2832233
GTTCTAG1356.942199E-410.0026481
GTATACA3800.09.8710341
GTATAAG3650.09.8656251
GTACTAT2957.712515E-109.6635751
CTTATAC3001.0550139E-99.49773
GTCCTGT1901.7515125E-59.4761921
TATACTG4750.09.4713915
CCTTTAC1603.13871E-49.3774821
TCTAGAC1603.1547097E-49.3727293
GTATATG5300.09.3420951
GTATAGA3705.456968E-129.3267941
Produced by FastQC (version 0.11.2)