FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_64.35200000039614.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_64.35200000039614.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137050
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19000.1670990721604151No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAG809.72863E-515.0042231
TAGGTAG1201.1247921E-613.7472185
CGTATCA902.6544728E-413.33180815
GCCGCGT800.001652333113.12927325
GTTCCGA954.1898436E-412.6306911
GGGCTCG850.002606720712.356421
TATACTG3650.011.5045225
TGTTCCG1059.719952E-411.42726510
GTCTTAT1901.3182944E-711.0557441
CTCACAC950.006007539611.049913
CTAATAC2651.09139364E-1010.75206853
GGCTAAG1551.9477704E-510.6481591
GTGTAAC1704.6593814E-610.5912161
GCTCAGA1000.00875868310.5029561
CGGGCGG1000.00878156210.49926111
TAGGGGT1000.00879015510.4978754
TAGTACA2156.4610504E-810.4629994
GTATTAG2905.0931703E-1110.347741
ATACAGC2352.1684173E-810.21076
GTATTAA2854.110916E-1010.0028161
Produced by FastQC (version 0.11.2)