FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_63.352000000395df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_63.352000000395df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730702
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21280.12295588726424307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCC750.00101935713.9980999
CTGTGCG750.00101935713.9980999
TAGACTG1651.8695573E-812.7251785
GGCTCGG1703.736768E-711.477321
CGATCAC1501.3584589E-511.00136724
CCTATAC2751.8189894E-1110.9079253
GTCCTAG1253.4046985E-410.8063391
CTAATAC4450.010.7853633
CCGCGCA1000.00878893310.4985759
GTGCTCC1000.00879081510.4982716
CGCACTC1150.002077008610.43396912
AGGTAGC1602.7802456E-510.3110997
ACTATGC4350.09.9986438
TGGACCC1952.2067015E-69.9983535
CAGGACC1501.6468023E-49.9983534
AGAGTGT3051.364242E-109.8344466
CTATACT3700.09.72812754
GTCCTGG1705.370831E-59.7115781
TACGTCT1409.7292534E-49.6412694
CTATGCA3801.8189894E-129.4723989
Produced by FastQC (version 0.11.2)