FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_62.3520000003959c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_62.3520000003959c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997561
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15930.1596894826481789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG1351.1077645E-915.5539445
GTATTAG2550.012.9463251
ACCCTGC1303.8444028E-511.5378448
TGTACTG1951.531589E-811.5372665
CCCTTAT1602.2489712E-611.2544761
CTGGGCA1355.6485685E-511.1105179
CACTCAG950.00600028711.0514885
TCTACAC1901.3246063E-711.0514883
CTAATAC2104.5651177E-810.7131763
GTACTAG1704.651052E-610.5924481
TAAGACA1851.1206994E-610.5394494
GCCTTAA1000.00874987610.5041771
TGAGGCC1150.00207525910.43422516
GACCGTC1150.002076124310.43370157
AGGTAGC1304.917798E-410.3835397
TCTATAC2651.2460077E-910.1876233
CAGTGCA1501.6433695E-49.9994659
ACTGGGC1356.9568394E-49.9994658
ATAGACT1356.960045E-49.9989644
AAGGTAG2454.127105E-89.7949046
Produced by FastQC (version 0.11.2)