FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_61.35200000039559.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_61.35200000039559.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398365
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25180.1800674358983527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTC750.001020453413.9957957
GTGCGAC1402.976958E-813.93335529
GTATTAG3400.013.67971
GGTGCGA1404.489939E-712.86155928
ACACGCA1551.1348493E-712.57911615
TGCGACG1501.0361036E-612.00326330
CCAGGAC1252.581559E-511.9968243
CTTACAG1406.3352945E-611.7825963
GTCCTAC900.00400076211.6694221
ACAGGCA2301.3187673E-911.0836278
CTAGGAC950.006009530711.0497063
CCTAGAC1100.001436187410.9062043
TAGGACT1100.001436187410.9062044
TAGGTAG1653.2839907E-610.9058145
CTAATAC3450.010.8666893
GTCCTAA1951.8893843E-710.7717731
GGGTATA1704.6644036E-610.5907351
GGGTAGG1704.6644036E-610.5907351
CAACACG1704.684396E-610.58694813
CCTAATA3850.010.5178252
Produced by FastQC (version 0.11.2)