FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_60.35200000039515.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_60.35200000039515.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632287
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8060.12747375796117902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG601.8617095E-417.5028151
ACTGGTC750.001019470713.9956088
ACAGTCC650.006395137413.841818
ACTGTCC902.6560336E-413.3291518
GGACAGT800.001658887313.1208836
CTAGAAC850.00261616912.3490653
CAAGACT850.00261616912.3490654
TCTATAC1354.2140673E-612.2183893
GTATAGA1901.3164208E-711.054411
TAGGTAG950.006005556311.0491655
CTAGTAC950.006005556311.0491653
GATCTAT950.006005556311.0491656
AGAAGTG1253.4266713E-410.7966125
GGATAGC1000.00879017110.4967066
TAGAACC1150.002077943910.431514
TATACTG2453.7216523E-910.4048995
TACACTG1451.1682169E-410.3415835
CCTATAC1602.7780568E-510.3092653
CTATAAT2352.1476808E-810.214411
GATTAAG1200.002946792210.0016091
Produced by FastQC (version 0.11.2)