FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_59.352000000394d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_59.352000000394d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072926
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11840.11035243809917926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAG902.6574824E-413.3298953
TAGACAG2051.4551915E-1113.1667215
GGCGAGT850.00261529512.35090612
CCTGTCC850.00261803912.3491798
GTATACG2257.094059E-1112.0019391
CCTATAC1652.5620284E-711.8151343
GACAGTC1151.5826905E-411.7355577
ACAGTCG900.004015913211.6631148
TGTTAGG1303.851235E-511.5360232
GGCTCGG1059.696728E-411.4304191
GAGAGTC1459.360274E-611.3758467
CCTATAG1355.6612604E-511.1082463
GTCGTAG950.00598895611.0544181
GTGTGCG950.006003518611.05081111
CTATACT2453.2014214E-1011.0170544
CTCCTAG1100.001430616510.9108531
GCTCGGA1100.001435076410.9067862
CTTATAG1551.9591354E-510.6424163
TCTGAGC1000.00879323810.4972923
AGGAGTG2002.6951238E-710.4968035
Produced by FastQC (version 0.11.2)