FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_58.3520000003949d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_58.3520000003949d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679469
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13440.19780151853874128No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCCG400.006519349818.7442516
GGACTGA500.001111874117.994486
TACCGTG859.218977E-615.8774837
ACTATGC1803.2923708E-1013.3292468
GTATTAG1701.9772415E-913.2390311
ATACCGT1804.6766218E-912.4961676
CAGCTGA850.002616376412.34915359
GTTACAC1151.581086E-411.7355323
TAAGACA1551.5456335E-611.6093434
GTACTAG1059.6789445E-411.4317981
AGAGGTC1059.7268086E-411.4250688
GGTTATA950.00597975411.0557511
TTAGACC950.00600602211.0492433
ACTACAC1100.001435043910.90574553
GATTAAT1253.4085396E-410.8030481
GTCTATA1408.1384875E-510.717311
TATACTG3101.8189894E-1210.6418975
ACTGTCA1000.0087477710.503736524
TCAGGCC1000.00879084410.4967799
CTGGCCA1000.00879084410.4967799
Produced by FastQC (version 0.11.2)