FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_57.3520000003945a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_57.3520000003945a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485123
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACC553.884248E-621.8137598
GACCGTG400.006512816518.7461997
AGTGCGC400.006512816518.7461998
GTATTAG1253.6925485E-1016.801791
GTATTAC809.724835E-515.0015981
TTAGGTA901.580839E-514.9969614
TCTTAAG952.6314206E-514.2091092
TAAGCAG851.6331843E-414.1147865
CAGACCC750.001018015613.9971629
GTATAAA1853.45608E-1113.7852521
GTATTAT2750.013.6378151
AGACCGT1106.9041507E-613.6335996
GGTAGTC1004.2686785E-513.4972637
TAGGTAG902.6515452E-413.3306315
TAGACAC800.00165139613.12775125
TACAGCG800.00165653313.1223397
TATCCAG800.00165653313.1223395
TCTATAC1507.365088E-812.9973643
GTAGTCA954.1868282E-412.6290188
TGTAGCA1201.6902199E-512.49875452
Produced by FastQC (version 0.11.2)