FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_56.35200000039416.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_56.35200000039416.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731416
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13730.18771807015433079No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCGA350.003006552321.42681310
GTCCTAA852.2051609E-819.4194661
CTAATAC2200.016.3622933
CCTCCGA600.003754231114.9987722
CTTAAGG809.7512544E-514.9987693
TAGACTA952.63459E-514.209365
TCCTAAC851.6314043E-414.118422
TAGGTAG750.001017955213.9988525
TACACGG650.006386592513.8450175
ACGGTGT1004.2696403E-513.4988938
CCTAATA1905.4569682E-1113.4217862
ATGCCTC902.6517222E-413.33223917
GCCTAGA800.001650467913.1302071
CCTAACA800.00165642813.1239223
GTTCAGG1151.0872021E-513.0486531
GGTAGCC954.187082E-412.6305438
TCCTAGT850.002609623112.3536172
ACCGTGT850.002612301412.3519288
GTATATA4600.012.07000351
TAAACCG1006.448473E-411.9990165
Produced by FastQC (version 0.11.2)