FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_55.352000000393c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_55.352000000393c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992191
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15710.15833644933284013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCTT706.0382136E-414.99921813
TCTAAGC1104.2077772E-714.9969513
TAGGGGT809.766921E-514.996954
TAGGTAG1503.0559022E-1014.9961965
AGAGTGT1251.1357042E-714.3963486
GACCTAT851.6331117E-414.1176231
CTTACAC750.001019338113.9971553
GAGGGCG750.001019726513.9964499
CTACCCC650.006393839613.8433394
CTAATAC2950.013.7260243
GTATTAG2450.013.4693641
TAGTCAG1456.9136513E-712.4106449
CTCGGTC850.002616800812.3498087
GTAGTCA1702.8341674E-812.3498088
GTATTAA2503.6379788E-1211.9999811
TAATACT4250.011.9975614
ATACTGT3650.011.9147856
AGACTGC1151.5817126E-411.7361536
AGGTAGT2052.566594E-911.7043496
GTGTAGC900.00400647311.6666471
Produced by FastQC (version 0.11.2)