FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_54.35200000039381.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_54.35200000039381.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270101
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21030.16557738321598045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGA400.006514615418.7481310
ACTGGTC1106.9178805E-613.6350038
AGACTGT1454.7433787E-813.4469356
TAGTCAG1601.2134478E-813.1236919
AGTGTGC800.001657819313.1236918
GTATTAG3800.013.0286061
CTAGGAC954.1914813E-412.6303193
CTAGCCT954.1914813E-412.6303194
TAGACGG1006.4551976E-411.9988035
TAGGTAG2301.05501385E-1011.737965
TATTAGG3700.011.3502192
CTAATAC2701.2732926E-1111.11000253
GGGCTCG950.00598934811.0545751
GGTAGTC2104.569847E-810.7132177
GTATTAT6000.010.5018471
GATTAGG1000.00878442310.4989532
GTACTAG1150.002071974610.4366181
TTATACT4050.010.3693364
GTATAGA3950.010.2549681
TTAGGTA2803.0013325E-1010.1775564
Produced by FastQC (version 0.11.2)