FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_53.3520000003934e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_53.3520000003934e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465843
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATATATACATGAGAAGAACGGCATAGTGCG7680.1648624107263606No Hit
TATATATACAGGCAACACGCAGATATAGGTGCGACG6290.13502403170166774No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5330.11441623036087264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCATC350.003006869721.424437
TCGGTGA350.003006869721.4244310
TAACACT601.8654269E-417.496624
TGCGACG1405.456968E-1217.14874830
GTATTAG1253.6925485E-1016.8003621
GGCATCC550.002101831616.3604748
GTGCGAC1451.8007995E-1015.52257429
GGTGCGA1604.9112714E-1115.00515628
GGACCAT706.036154E-414.9971026
TCCGCTC851.625402E-414.122498530
TAGACAG650.006386704713.8434785
AGGTGCG1651.2587407E-913.64104927
TCGCTCC1004.2460942E-513.5046428
GGCTATA902.6461636E-413.333621
AAGTGTC800.001656242213.1224637
ACACAGT954.1859367E-412.6291386
TGAGACT954.1859367E-412.6291384
TCGCCAT850.002603620812.35585924
TGATGTG850.002612023412.35055355
GGCAACA1951.1568773E-912.30663411
Produced by FastQC (version 0.11.2)