FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_52.35200000039317.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_52.35200000039317.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884847
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14310.1617228741240011No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGCG500.001108265718.0040724
CCTAGCG706.024565E-415.00339230
CGATCAC706.024565E-415.00339224
TAGGTAG1206.879236E-814.9974575
ACGGTGT1507.38346E-812.9985318
CTAGACA1056.757074E-512.8549624
TATTAGG2950.012.7104272
ACGATCC954.1897173E-412.6301529
GTATTAG2102.8740033E-1012.1462891
TAATACT4950.012.1191574
GGAGTGT1151.5801039E-411.7371416
ATACAGG2701.8189894E-1211.6646896
CACCTAG950.00598467511.05513128
CCAGGAC950.00600232411.0507583
GTATAAT3900.010.7722761
CTTATAC1951.8965329E-710.76740553
GTATAGA3101.8189894E-1210.648171
ACCTAGC1000.00876337610.501780529
CGTCCAT1000.00878175510.49881410
TCTAGGA1150.00207588810.4336032
Produced by FastQC (version 0.11.2)