FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_51.352000000392d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_51.352000000392d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478142
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21320.14423512761290863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG1905.4569682E-1113.4195235
CGTATCC1056.759509E-512.8556793
GTATTAG3900.011.9269651
CTAATAC4650.011.2890373
GTGCGAC1355.6349214E-511.11398129
TACACTG1951.898843E-710.7680045
GTCCTAA1551.9479017E-510.6486321
TAGACTG1704.6845526E-610.5870295
GTAGACT1704.6845526E-610.5870294
TGCGACG1451.16427844E-410.3471530
GGTAGTC2352.168963E-810.2112587
CCTTAAG1952.1920769E-610.0032611
CCCCTAT1200.002946741210.0032611
ATACTGT5200.09.8062446
GTACTAT4450.09.7784681
GTTTAGG1705.3847656E-59.7090471
CTAGACT1705.410795E-59.7047774
GTCTAAT3101.8553692E-109.6805741
TAGGACC1250.00414533359.59890654
GACCATC1901.759449E-59.4722847
Produced by FastQC (version 0.11.2)