FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_50.3520000003929f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_50.3520000003929f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860213
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13700.15926288024012658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGC2800.013.925288
GTCCTAC650.006369750513.8509321
CGTGTTC650.0063844913.84610122
TAATACT4200.013.569014
CTATGCA2550.013.5262149
ACGCTCA902.6560386E-413.3301828
TAGACAG954.1938716E-412.62859255
CTAATAC2800.012.3192323
TATACCG1101.04461295E-412.2698265
ATCACAC2001.724402E-912.00344329
ACCGTCT1006.458883E-411.9971648
TCACACA2154.2564352E-1011.86317830
GTATTAG2701.8189894E-1211.6706921
GTACATG1303.8273563E-511.5424431
TACACAG1303.8501523E-511.5357345
GTGTAAC1202.3353133E-411.2538821
TTAATAC2402.2191671E-1011.2479953
CTGGGTG950.006005084611.0500189
TCTTATA2053.2012395E-810.9742922
TAATACC2607.6397555E-1110.9595844
Produced by FastQC (version 0.11.2)