FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_49.35200000039268.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_49.35200000039268.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1769045
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28540.16132998312648913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC550.002104112616.3614648
GCGCACT1151.0952366E-513.04174611
GTATTAG4550.012.5307671
TAATACT6300.011.4273764
GTCGGGG1059.7238313E-411.42737615
TAGGTCG950.006004705611.0511635
GAGCGGC950.006004705611.0511637
TAGACTG1653.2790067E-610.9076425
TAGGACG1253.4266195E-410.7988714
GTATTAA3750.010.4027351
GGTAGTC2651.2514647E-910.18732557
GTACTAG2701.717126E-910.0026291
TACACCG1653.9009938E-59.99867255
AGCGCAC1653.902206E-59.99838910
GAGGCCG1552.2907778E-49.6761349
GCTCCGC1250.0041466659.5987246
TAGTCAG2209.93492E-79.5441879
TAGAACC3001.0531949E-99.4990074
GCTATAC1757.432705E-59.4275863
CTTATAC3051.4206307E-99.3432863
Produced by FastQC (version 0.11.2)