FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_48.35200000039225.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_48.35200000039225.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264675
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4420.1669972607915368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGCT200.005140817729.9992457
GCCGCGT402.4609725E-422.49943425
TCGCCGC455.45223E-419.99949623
GTATAAC559.504284E-519.1012551
TCTTAAG559.5319185E-519.0940362
GCAGTGC400.006498512318.7495278
ATACGCT400.006498512318.7495278
TACAGGC400.006498512318.7495277
TTAGGTA500.001107343617.9995484
CGCCGCG500.001107343617.99954824
GTATTAG854.6576497E-717.6566221
AGGCGAA550.0020962516.36322419
ACTCCGC653.4247895E-416.1534398
GTATTAA809.662345E-515.0081291
TAAACAT901.5728088E-514.9996225
TGTCCTT600.003744389214.99962222
GCGTCGC952.620676E-514.21016820
AACTCCG851.626306E-414.1172927
CCGCATA750.001014506913.99964611
ATACAGG750.001014506913.9996466
Produced by FastQC (version 0.11.2)