FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_47.352000000391e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_47.352000000391e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519781
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6230.11985817103741768No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGT455.4794725E-419.9899967
TAGGTAG809.7694E-514.9939385
CTGCTTG809.7774166E-514.99249659
TCTTGGG954.1948393E-412.6264752
GTCCAAG850.00260931612.3527031
ATTACAC950.00600715911.0481663
TCCTATA1100.00143522610.9046832
ACAGCTC1000.00879880310.4947478
CAATACT1304.922924E-410.380424
GTGTTAG1200.00294968449.9998071
TCAATAC1952.2023432E-69.9959593
TACAACT1501.6457458E-49.9959594
AGAACTA1552.2900074E-49.673515
GTATATA5400.09.44426251
CTCTTAA1757.39868E-59.42839051
AATAGAG1603.150217E-49.3712115
TGAACAG1450.00134059539.3065825
ATACACA1450.00134059539.3065824
AATAGTG1300.00573035249.2270395
AACAGGT1300.0057348919.2261517
Produced by FastQC (version 0.11.2)