FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_46.352000000391ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_46.352000000391ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356109
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6600.18533651213532937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCCA200.0051391330.00407226
TAGTACT601.8631847E-417.497464
GTTCTAT653.406461E-416.1673931
GTATAGG705.984163E-415.012581
GTCTAGG600.00372630215.0125791
GTCTAGT750.001009106814.011741
AGAAGTG650.00638077613.8441455
ACAAGGC650.00638077613.8441458
TCTAGTA1004.254507E-513.4999372
GTATACG902.625202E-413.3445151
TTATAAC1151.5746703E-411.7374273
CAAGATA1459.294932E-611.3776594
ATAGTGC950.005983052311.05258129
TTAGAGT1100.001430207910.9075084
TATACCT1100.001430207910.9075084
TCTATAT1653.255538E-610.9075083
CAATACT1253.4134102E-410.7984334
TCTATAC1253.4134102E-410.7984333
GTATAAG1000.00870285510.5088051
TCAATAC1000.00876655310.4984763
Produced by FastQC (version 0.11.2)