FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_45.35200000039169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_45.35200000039169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243741
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC504.584273E-520.9995693
AGCGCTC400.006496895618.7496158
GTCTTAG500.001102418.0107171
ATTAACC550.002095543516.36333
ATACTAG809.703776E-514.9996936
TAATACT1251.7604816E-613.1997294
GTATATG1056.6701075E-512.8647981
AATACTA1201.6807106E-512.4997435
ATTATAC1006.418002E-411.9997543
GCAAAAA1151.5689433E-411.73888913
GTATAGA900.00397389511.6736121
CATTATA900.0039922311.6664272
TTCTATA1202.3301676E-411.2497692
ATTAGAG1100.00142591610.9088673
ATATTAC1150.002054476910.4409951
TAATAGT1150.002065031610.4345674
ATAATAC1150.002065031610.4345673
ATAATAG1451.1580549E-410.3446153
TATAATA1901.7377693E-59.473492
TATAACA1300.0056940439.230584
Produced by FastQC (version 0.11.2)