FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_44.35200000039119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_44.35200000039119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388870
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5480.1409211304549078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGCGT350.002999781221.43206230
GCACAGT350.003004261521.4265526
ACCGTGC400.00650647318.748238
ACCTAAT904.242611E-818.336321
CCCTGCC500.001109129917.9983029
CCCTATA500.001109129917.9983022
TGCACAG601.8630171E-417.498355
CCGTCGC550.002099602516.3620939
GTATTAA1203.8726284E-916.2526471
GTCTAGG706.016689E-415.0024441
CTCCTAT600.003743912815.0024441
ATTAGGT706.0284475E-414.9985863
CCTAATA1104.183512E-714.9985862
AAGCGAT600.003750326314.9985866
TATAATA1604.9112714E-1114.9985852
CCTATAC851.6303665E-414.1163173
CTATACT750.001016543513.998684
CCTGCAC650.006380019213.8448493
CTAATAC1301.82039E-713.8448493
ATATGAC1201.1179727E-613.7487043
Produced by FastQC (version 0.11.2)