FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_43.352000000390d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_43.352000000390d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299269
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4360.1456883272240025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCT300.001224872924.9984669
AATCAGT559.553719E-519.089746
ACCGATT400.00650180918.748858
TTCTATA500.001108072217.9988982
TAATCAG601.8607374E-417.4989265
ATCAGTG653.427604E-416.1528577
GTATTAA600.003734331815.0066021
GTGCTAG600.003734331815.0066021
TAGCACT600.003746813914.999084
GTATGCT750.001015328113.9991434
AGACAGA800.001652191313.1241956
ATAAATC954.1735207E-412.6308053
CATATAA1006.400704E-412.0052821
TAAAGGT900.003997084711.6659534
TCCAATA1202.3344347E-411.24931052
ATGTAAG950.00595950411.0574971
TATCCTG950.005981793611.0519545
GTATATA1000.0087231210.5046221
AGAACAT1000.00875561910.4993565
TATACCA1200.00294447979.9993874
Produced by FastQC (version 0.11.2)