FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_42.35200000039091.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_42.35200000039091.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804753
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15150.1882565209449359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCGT550.00209817516.36682325
CCCGGAT550.002099046516.36580524
CCGGATC650.006375625713.8488525
CGCCGCG650.00637825113.84798924
GTATAGA2500.013.20585251
TTAAGAC1151.09328375E-513.04196553
CGGTGTT1302.7590522E-612.6908359
ATACGGT1907.730705E-1012.6301156
TGTACAC1702.8256181E-812.3515083
GTATTAG1951.1532393E-912.313151
ACTCTGC900.004008771411.6653146
ACACTCC1059.7146945E-411.4272458
GTACTAG1202.3328283E-411.254991
GTAGCGC950.005999133511.0513516
CAGTCTC950.005999133511.0513519
GGCTATT950.005999133511.0513517
GTCTTAG1100.001426584110.9139291
ATAGAAC2251.0984877E-810.665433
TACGGTG2002.684519E-710.4987817
CATAGCC1000.00878079110.4987813
Produced by FastQC (version 0.11.2)