FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l02n02_ntr_41.3520000003905e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l02n02_ntr_41.3520000003905e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674645
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGT200.005148566329.9966664
GAGCCGC400.00651195118.7479179
GACCGTC653.4368946E-416.152057
CGTCTCA600.003754556614.99833310
CGTCGCT600.003754556614.99833310
ACCGTCG600.003754556614.9983338
TTAGGGA706.037372E-414.9983314
CCGTCGC650.006387147613.8446149
CCTATAC1151.0926064E-513.0420283
CTCCTGA1056.725354E-512.8604791
TATACCC1201.6927372E-512.49861053
TGGACTG850.002612509812.3515685
CTGGACA850.002612509812.3515684
GTCTAGA1006.428847E-412.0031141
TAACACA1252.572866E-511.9986674
GTACTAT900.003996394611.6696941
ACTGATC900.004007478711.6653698
CAGGGCT900.004007478711.6653699
ACAGGTC900.004007478711.6653698
TAAGGAG1551.5419446E-611.6116125
Produced by FastQC (version 0.11.2)